In Silico Identification of the Full Complement of Subtilase-Encoding Genes and Characterization of the Role of <i>TaSBT1.7</i> in Resistance Against Stripe Rust in Wheat

Plant subtilases (SBTs) or subtilisin-like proteases comprise a very diverse family of serine peptidases that participates in broad spectrum biological functions. Despite increasing evidence for roles SBTs plant immunity recent years, little is known about wheat (Triticum aestivum) (TaSBTs). Here, we identified 255 TaSBT genes from bread using the latest version 2.0 reference genome sequence. The SBT can be grouped into five clades, TaSBT1 to TaSBT5, based on phylogenetic tree constructed with deduced protein sequences. In silico protein-domain analysis revealed existence considerable sequence diversification which, together local clustered gene distribution, suggests have undergone extensive functional diversification. Among those whose expression was altered by biotic factors, TaSBT1.7 found induced leaves chitin and flg22 elicitors, as well six examined pathogens, implying role defense. Transient overexpression Nicotiana benthamiana resulted necrotic cell death. Moreover, knocking down barley stripe mosaic virus-induced silencing compromised hypersensitive response resistance against Puccinia striiformis f. sp. tritici, causal agent rust. Taken together, this study defined full complement provided positive one particular member, TaSBT1.7, incompatible interaction between rust pathogen.